FB2 Group

Research Lines


The basic objective of the research group is the study of basic physiological mechanisms in fish related to metabolism, neuroendocrinology, rhythms, stress response and mechanisms of adaptation to the environment. We use model fish species (rainbow trout, flounder, zebrafish ...) in order to extrapolate the results to the development of applied research in the field of aquaculture.

At present, the main field of work of the group is focused on the study of metabolic, neuroendocrine and environmental mechanisms involved in the control of feeding in fish and their interaction with stress.


Research Lines:

  • Control of food intake in fish by metabolic, neuroendocrine, and environmental factors.
  • Nutrient sensing mechanisms and energy metabolism in fish.
  • Interaction between mechanisms controlling food intake in fish and stress response.
  • Physiology of the pineal gland and melatonin.
  • Daily and seasonal rhythms in fish. molecular mechanisms of the circadian clock.
  • The hypothalamus-pituitary-interrenal axis and stress response in fish.

Fish Models


Rainbow trout

Rainbow trout

Oncorhynchus mykiss
Senegalese sole

Senegalese sole

Solea senegalensis
Gilthead sea bream

Gilthead sea bream

Sparus aurata
Turbot

Turbot

Scophthalmus maximus
Zebrafish

Zebrafish

Danio rerio

Resources


EQUIPMENT

  • UV-VIS spectrophotometer with multiple cuvettes, thermostatic control and continuous stirring.
  • UV-VIS plate reader.
  • Fluorimeter.
  • HPLC system with electrochemical detection.
  • HPLC system with UV detection.
  • HPLC system with fluorescence detection.
  • Automatic sample injector for HPLC systems.
  • Conventional PCR system (thermocycler, electrophoresis system, system of image analysis for gels) to assess gene expression.
  • Real-time PCR (qPCR) to quantify gene expression.
  • Western blot system (buckets, power supply, etc.) to quantify levels of specific proteins.
  • Vacuum concentrator.
  • Equipment for basic immunoshistochemistry (microtome, microscope, etc.).
  • Microcentrifuge, refrigerated centrifuge and ultracentrifuge.
  • Basic laboratory -Equipment: -20 and -80 freezers, distillation and ultra-pure water equipment, balances, pH meters, normal and orbital shakers, incubation baths, incubation stoves, sterilizer, sonicator, homogenizer, micropipettes, multichannel pipettes, dispensers, etc.

FACILITIES

  • 2 Equipped research laboratories (100 m2 in total).
  • Cell culture laboratory (CO2 incubator, strelizer, laminar flow hood, inverted microscope).
  • Wet lab for fish experimentation in Marine Science Station (ECIMAT) of the University of Vigo in Toralla Island.

TECHNIQUES

  • Techniques of manipulation, surgery, substance delivery, collection and processing of samples for research both in vivo and in vitro with tissues and cells of fish.
  • Quantification of metabolites and enzymatic activities by UV-VIS spectrophotometry, HPLC, and fluorimetry.
  • Quantification of neurotransmitters and hormones.
  • Quantification by qRT-PCR of mRNA levels resulting from gene expression.
  • Quantification by western blot for the presence of specific proteins.
  • Quantification by ELISA of plasma hormone levels.
  • Techniques of immunohistochemistry to assess the presence of specific proteins in tissues.
  • Evaluation of parameters related to food intake and growth.

Grants supporting research


Integrative regulation of food intake in fish by metabolic, circadian, and neuroendocrine factors. Implications for pisciculture
01/01/2017 - 12/31/2019 | Spanish Ministerio de Economía y Competitividad | Ref. AGL2016-74857-C3-1-R
Main researchers: José Luis Soengas Fernández and Jesús Manuel Míguez Miramontes.

Thematic Network on Fish Welfare and Stress
01/01/2017 - 12/31/2019 | Spanish Ministerio de Economía y Competitividad | Ref. AGL2016-81808-REDT
Main researcher: José Luis Soengas Fernández.

Integration of metabolic and neuroendocrine signals in the regulation of food intake and its interaction with stress in fish
01/01/2014 - 06/30/2017 | Spanish Ministerio de Economía y Competitividad | Ref. AGL2013-46448-C3-1-R
Main researchers: José Luis Soengas Fernández and Jesús Manuel Míguez Miramontes.

Consolidation and structuring of competitive research units of the Galician Univeristy system (Group with growth potential)
06/16/2012 - 11/30/2014 | Spanish Xunta de Galicia | Ref. CN 2012/004
Main researcher: José Luis Soengas Fernández.

Analysis and evaluation of different methods of slaughter of marine fishes.
01/01/2011 - 12/31/2012 | Spanish Ministerio de Medio Ambiente y Medio Rural y Marino
Main researcher: Jesús Manuel Míguez Miramontes.

Influence of intrinsic signals (metabolic sensors and circadian system) and stress on the control of food intake in teleost fish. Use in fish culture.
01/01/2011 - 06/30/2014 | Spanish Ministerio de Ciencia e Innovación | Ref. AGL2010-22247-C03-03
Main researcher: José Luis Soengas Fernández.

Consolidation and structuring of competitive research units.
01/01/2010 - 12/21/2010 | Spanish Xunta de Galicia | Ref. IN845B-2010/162
Main researcher: José Luis Soengas Fernández.

Gastrointestinal disturbances arising from aging in the circadian system and melatonin production at gastrointestinal and hepatic levels. Characterization of clock genes and protective role of melatonin.
06/30/2009 - 07/01/2011 | Fundación Mutua Madrileña
Main researcher: Marcos Antonio López Patiño.

Consolidation and structuring of competitive research units.
01/01/2009 - 12/16/2009 | Spanish Xunta de Galicia | Ref. INCITE09ENA310002ES
Main researcher: José Luis Soengas Fernández.

Regulation of food intake in teleost fish by metabolic and neuroendocrine factors. Effect of stress
12/01/2007 - 11/30/2010 | Spanish Ministerio de Educación y Ciencia | Ref. AGL2007-65744-C03-01/ACU
Main researcher: Jesús Manuel Míguez Miramontes.

Rhythmicity and neuroendocrine regulation of food intake in teleost fish.
07/13/2005 - 07/12/2008 | Spanish Xunta de Galicia | Ref. PGIDIT05PXIC31202PN
Main researcher: José Luis Soengas Fernández.

Effects of exposure to polycyclic aromatic hydrocarbons on reproduction and its neuroendocrine control in teleost fish.
12/17/2004 - 12/16/2007 | Spanish Xunta de Galicia | Ref. PGIDT04PXIC31208PN
Main researcher: José Luis Soengas Fernández.

Rhythmicity and neuroendocrine regulation of food intake in teleost fish.
12/16/2004 - 12/15/2007 | Spanish Ministerio de Educación y Ciencia | Ref. AGL2004-08137-C04-03/ACU
Main researcher: Jesús Manuel Míguez Miramontes.

Effects of exposure to polycyclic aromatic hydrocarbons on reproduction and its neuroendocrine control in teleost fish.
12/17/2003 - 12/16/2006 | Spanish Ministerio de Ciencia y Tecnología | Ref. VEM 2003-20062
Main researcher: José Luis Soengas Fernández.

Interaction between osmoregulation and energy metabolism in gilthead sea bream.
12/28/2001 - 12/27/2004 | Spanish Ministerio de Ciencia y Tecnología | Ref. BOS2001-4031-C02-02
Main researcher: José Luis Soengas Fernández.

Study of neuroendocrine mechanisms involved in adaptation to different environmental salinities in teleost fish using rainbow trout as a model.
07/19/2001 - 07/19/2004 | Spanish Xunta de Galicia | Ref. PGIDT01PX130113PR
Main researcher: José Luis Soengas Fernández.

Characterization of the hormonal regulation of brain energy metabolism in rainbow trout (Oncorhynchus mykiss).
10/10/1999 - 10/09/2001 | Spanish Xunta de Galicia | Ref. PGIDT99PX130106A
Main researcher: José Luis Soengas Fernández.

Thematic networks


NATIONAL


PISCIBIEN. Thematic Network on Fish Welfare and Stress

Formed by the Fish Physiology Research group of the University of Vigo, and Research groups from: Automous University of Barcelona (leader), University of Cádiz, Polytechnic University of Madrid, University of Murcia, Aquaculture Institute Torre de la Sal-CSIC, Complutense University of Madrid, Institut de Recerca i tecnología Agroalimentaria (IRTA), University of las Palmas de Gran Canaria, and Instituto de investigación y formación agraria y pesquera de Andalucía (IFAPA)

INTEGRAFISH. Metabolic, circadian, and behavioral integration of food intake control in fish

Formed by the Fish Physiology Research group of the University of Vigo (leader), and Research groups from:  Complutense University of Madrid (María Jesús Delgado) and Aquaculture Institute Torre de la Sal-CSIC (José Miguel Cerdá-Reverter)

INTERNATIONAL


CLEANFISH. Thematic Network on Fish Welfare, growth, and metabolism

Formed by the Fish Physiology Research group of the University of Vigo, and Research groups from:  Centre of Neurosciences and Cellular Biology of Coimbra-Portugal (leader), INRA StPée-France, Wageningen University-The Netherlands, University of Porto-Portugal, Norwegian University of Science and Technology-Norway, Silico Life Ltd., and University of the Basque Country-Spain

Collaborations


SPANISH GROUPS

INTERNATIONAL GROUPS

Training of researchers (PhD Thesis)


FINISHED

Circadian system and hepatic metabolism in rainbow trout. Influence of stress
07/22/2016 | Juan Hernández Pérez | Supervisors: Jesús M. Míguez and Marcos A. López Patiño

Central and peripheral fatty acid sensor mechanism in rainbow trout as a model of teleost fish. Involvement in the control of food intake
06/18/2015 | Marta Librán Pérez | Supervisor: José Luis Soengas
International PhD Mention | Special awarded PhD Thesis
.

Effects of stress on food intake and its regulation by neuroendocrine and metabolic mechanisms in teleost fish
10/16/2012 | Marta Conde Sieira | Supervisors: José Luis Soengas and Jesús M. Míguez
International PhD Mention | Special awarded PhD Thesis
.

Melatonin in the gastrointestinal tract of rainbow trout: regulation and physiological role
09/30/2011 | José Luis Muñoz Pérez | Supervisors: Jesús M. Míguez and Marcos A. López Patiño

Role of leptin in the relationship between central glucosensor systems and the control of food intake in rainbow trout as a teleost fish model
09/27/2011 | Ariel José Aguilar | Supervisor: José Luis Soengas

Regulation of metabolic and secretory activity of the pineal organ of the rainbow trout by arginine vasotocin: interaction with osmotic adaptation
07/30/2008 | Arnau Rodríguez Illamola | Supervisor: Jesús M. Míguez

Regulation of indole metabolism and synthesis of melatonin in the pineal organ of the rainbow trout by photoperiod and feeding
07/09/2008 | Rosa María Ceinos Caride | Supervisor: Jesús M. Míguez

Effects at neuroendocrine level of exposure to polycyclic aromatic hydrocarbons in rainbow trout (Oncorhynchus mykiss)
07/03/2008 | Manuel Gesto Rodríguez | Supervisor: Jesús M. Míguez

Glucosensing mechanisms in brain and Brockmann bodies of rainbow trout as teleost fish model: physiological implications
06/18/2008 | Sergio Hugo Polakof Requejo | Supervisor: José Luis Soengas
European PhD Mention | Special awarded PhD Thesis

Effects of exposure to polycyclic aromatic hydrocarbons on energy metabolism and levels of steroid hormones in teleost fish
02/16/2007 | Adrián Tintos Gómez | Supervisor: José Luis Soengas

Interrelationship energy metabolism and osmoregulation in teleost fish. Hormonal control and interaction with other physiological processes
05/06/2005 | Susana Sangiao Alvarellos | Supervisor: José Luis Soengas
Special awarded PhD Thesis


FORTHCOMING

Role of brain monoaminergic systems (catecholamines and serotonin) on feeding behaviour and stress influence on teleost fish
Mauro Chivite Alcalde | Supervisor: Jesús M. Míguez

Role of fatty acid sensor mechanisms in the regulation of food intake in rainbow trout as a model of teleost fish: endocrine control and intracellular mechanisms
Cristina Velasco Rubial | Supervisor: José Luis Soengas

Cricadian organization and central modulators of food intake in trout. Role of melatonin and serotonin
Fatemeh Naderi | Supervisors: Jesús M. Míguez and Marcos A. López Patiño

Advances in the study of glucosensing systems in rainbow trout and their involvement in the control of food intake
Cristina Otero Rodiño | Supervisor: José Luis Soengas